Germinating Peas Cellular Respiration Lab

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BIO 101 Lab 07: Measuring Cellular Respiration and Fermentation

Notification: If you have a disability that makes information technology difficult to complete this lab, please contact

your instructor. Please provide your instructor a re-create of the Memorandum of Accommodation

(MOA) from NVCC Disability Support Services.

Objectives:____________________________________________________________

Distinguish between anaerobic and aerobic respiration.

Measure out the rate of oxygen consumption by living organisms during aerobic cellular

respiration.

Calculate metabolic rate from the experimental data.

Measure the rate of yeast fermentation.

Groundwork:___________________________________________________________

All life requires energy to survive. The energy of glucose is used as fuel by nearly all living

organisms to stay alive, to maintain homeostasis, and run all kinds of life activities. The

energy stored in chemical bonds of glucose and other food molecules needs to exist released

and converted into a diffusible, usable course ATP. If a terminal electron acceptor (such as

oxygen, sulfur, or a metallic ion) is present, then cellular respiration will occur. Aerobic

respiration uses oxygen every bit the concluding electron acceptor while anaerobic respiration uses

a diverseness of terminal electron acceptors other than oxygen. Both aerobic and anaerobic

respiration use electron send chains to produce ATP via oxidative phosphorylation. In the

absence of a terminal electron acceptor, instead fermentation will occur. In fermentation, the

electrons are recycled via the NAD+/NADH coupling and ATP is produced simply by substrate

level phosphorylation.

Aerobic respiration tin can produce upward to 38 ATPs per molecule of glucose equally compared to the

two ATP that are generated by fermentation. The three major parts of aerobic respiration are 1)

Glycolysis, 2) Krebs Bike (Citric Acrid Cycle), and three) Electron Transport Chain (ETC) and

oxidative phosphorylation. The overall equation of cellular respiration in well-nigh cells is:

C6H12Ohalf dozen + 6 Oii → half-dozen CO2 + 6 H2O + (32-38) ATP

We can measure the charge per unit of cellular respiration by measuring the consumption of the

reactants (glucose or oxygen), or by measuring the rate of product of the cease products

(carbon dioxide or water) of this procedure. The easiest component to measure is oxygen

consumption. If the living organism isouth placed in a airtight system with the carbon dioxide

removed as it is produced, then the consumption of oxygen can be determined using a

respirometer. The respirometer consists of a glass container sealed with a prophylactic stopper

outfitted with a pipette. A compound such as potassium hydroxide is placed in the

respirometer to remove the carbon dioxide generated. As the living organism within

consumes oxygen, water will displace the gas in the pipette and the volume of oxygen

consumed can exist measured over time to calculate the rate of cellular respiration and

metabolic charge per unit.

BIO 101 Lab 07: Measuring Cellular Respiration and Fermentation ane

Germinating Peas Cellular Respiration Lab,

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